Antibodies and other immunoreagents are so tied to lateral flow assays that we frequently use the terms LFA (lateral flow assay) and LFIA (lateral flow immunoassay) interchangeably. However, lateral flow assays may have no immunoreagents whatsoever onboard.
This article highlights the importance of designing a rapid diagnostic test for its intended purpose. Before going into the lab to develop an assay, one must start at the end and correctly identify the goals and use of the product. Only then can a point of care diagnostic device be designed correctly.